Biological control of nematodes In Vitro root-knot nematode (Meloidogyne sp.) Based on chitosan and fluensulfone
DOI:
https://doi.org/10.47808/revistabioagro.v4i1.17Keywords:
nematode, mortality, biopolymer, efficacy, chitosanAbstract
The root-knot nematode (Meloidogyne sp.) has potential economic importance in several crops, in his battle almost always used different agrochemical products, so we need to evaluate new products that are a better alternative in handling and caring for the environment. In this paper the biological effectiveness of different extracts of natural biopolymer chitosan with nematicide and regulatory effect of populations of these plant parasitic nematodes was evaluated, and Fluensulfone a nematicide broad spectrum, The in vitro study was conducted, soil samples were collected and the nematodes were extracted by the method of Baermann funnel, were placed in petri dishes in dilutions with 30 to 35 nematodes per experimental unit, where for control was used chitosan extracted river shrimp and commercial chitosan at different concentrations (0. 25, 0.50, 0.75), also a commercial nematicide extract chitosan Nematrol Plus, this compared with other commercial nematicides DiTera (Myrothecium verrucaria) Nemover (Extract pine) and Nimitz (Fluensulfone). It was found that the effect of chitosan nematicide medium dose (0.50) was better after 24 hours after application with a mortality of 100% 95.83 nematode, and an efficiency of 100%, which compared with the commercial nematicides.
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References
Aballay, E. 2005. Uso de plantas antagónicas para el control de nematodos fitoparásitos en vides. (On line) Facultad de Ciencias Agronómicas, Universidad de Chile. Santiago, Chile. Consultado en <http://mazinger.sisib.uchile.cl/reposit orio/lb/ciencias_agronomicas/monteale gre_j/19.html>. (Consultado el 17 de Septiembre de 2014).
Agrios, G., N.1996. Fitopatología. 2a ed. Ed. Limusa. México: 696-697.
Araus, C.L.F. 1998. Fitopatología un enfoque agroecológico. 1ª edición. Editorial de la Universidad de Costa Rica. San José Costa Rica. 245p.
Araya, A. y A. Cheves 1997. Efecto de cuatro nematicidas sobre el control de nematodos en banano (Musa AAA). Corbana 22 (47): 35-48.
Benjakul, S., Visessanguan, W., Tanaka, M., Ishizake, S., Suthidnham, R., and Sungpech, O. 2000. Effect of chitin and chitosan on gelling properties of surimi from barred garfish (Hemiramphus far). Journal of the Sciense of Food and Agriculture (81):102-108.
https://doi.org/10.1002/1097-0010(20010101)81:1<102::AID-JSFA792>3.0.CO;2-O
Bridge, I. y Williams, T.D. 2002. Plant Parasitic Nematodos. In: Waller, M; Lenne, I.M. y Waller, S.I. (eds). Plant Pathologist's Pocket book. Londres, CAB Internacional:140-162.
https://doi.org/10.1079/9780851994581.0140
Csinos, A, J. Whitehead, L.L. Hickman, S.S. LaHue. 2010 Evaluation of fluensulfone for root knot nematode on tobacco, Phytopathology (28): 100.
Dávila, M.N. Acosta C. Betancourt y J. Negron. 1999. Capacidad quitinolitica de hongos aislados de suelos agrícolas infestados con el nematodo nodulador (Meloidogyne spp.) en Puerto Rico. J. Agric. Univ.P.R. 83 (3-4): 189-199.
https://doi.org/10.46429/jaupr.v83i3-4.3889
Hoagland, P.D., and Parris, N. 1996. Chitosan/pectin laminated films. Journal of Agricultural and Food chemistry 44:1915-1919.
https://doi.org/10.1021/jf950162s
Kearn, J.; Ludlow, E.; Dillon, J.; O'Connor, V.; Holden-Dye, Lindy. 2014. Fluensulfone is a nematicide with a mode of action distinct from anticholinesterases and macrocyclic lactones. Pesticide Biochemistry and Physiology: 44-57.
https://doi.org/10.1016/j.pestbp.2014.01.004
Lafontaine. P.J., and Benhamou, N. 1996. Chitosan treatment: An emerging strategy for enhancing resistance of greenhouse tomato plants to infection by Fusarium oxysporum f.sp. radicis- lycopersici. Biocontrol Science and Technology 6:11-24.
https://doi.org/10.1080/09583159650039575
Peña, F. R., 2003. Actividad nematicida in vitro de formulaciones quitosan-L. tridentata (D.C.) Coville contra el nematodo falso nodulador Nacobbus aberrans (Thorne, 1935) Thorne y allen, 1944. Tesis de Licenciatura. Universidad Autónoma Agraria Antonio Narro UAAAN. Coahuila México: 33-40.
Sánchez, P.J.F. 2010. Efecto de quitina y quitosano sobre huevos y juveniles de nematodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incógnita. Bajo condiciones in vitro e in vivo. Tesis de Maestría.
Sandford, P. 1989. Chitosan: Commercial uses and potential applications: 51-69.
Sasanelli, N. 1992. Nematicidal activity of aqueous extracts from leaves of Ruta graveolens on Xiphinema index. Nematología Mediterránea. 20: 53-55.
Shahidi, E, Kamil, J.K., Jeon, Y., and Kirn, S. 2001. Antioxidant role of chitosan in a cooked cod (Gadus Morhua) model system. Journal of Food Lipids 9:57-64.
https://doi.org/10.1111/j.1745-4522.2002.tb00208.x
Y. Oka, S. Shuker, N. Tkachi, Nematicidal efficacy of MCW-2, a new nematicide of the fluoroalkenyl group, against the root-knot nematode Meloidogyne javanica, Pest. Manag. Sci. 65 (2009):1082-1089.
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Copyright (c) 2016 A.M García Munguía, R.N Contreras Martínez, C.A Ramos Barreto, M. Pérez Sato, W.K Gastélum Ferro, L.E Pérez Cabrera, A. Ibáñez Martínez, C.A García Munguía
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