In vitro propagation of Platicerium andinum Baker from spores
DOI:
https://doi.org/10.47808/revistabioagro.v8i2.193Keywords:
gaemtophyte, haploid spores, in vitro cultureAbstract
The forests of San Martin Region, habitat of Platycerium andinum B. have been destroyed in an excessive way, caused by anthropogenic activities, which has led to the loss of the species in its natural habitat, adding to it the extraction for its exuberant beauty to be market as an ornamental plant, its spores are also difficult to germinate under natural conditions, in addition, there is no methodology for the in vitro propagation of this species. The objective of this research is to determine the adequate concentration of sodium hypochlorite to obtain pathogen-free P. andinum spores and to evaluate three farming media to determine the most suitable medium for the propagation of gametophytes. The spores were obtained from the fertile fronds of adult plants by scraping them off. The germination of the spores was evaluated after 10 days, time in which they began to germinate and after 30 days there was already abundant gametophyte tissue; it was evaluated through the spore germination rate (GI) using the Braun-Blanquet abundance-coverage scale, reaching scale 5 after 60 days (any number of gametophytes with coverage greater than 75%). Regarding the determination of the best farming method for the in vitro propagation of gametophytes, three MSB media (T1, T2 and T3) were used with additives of 0.4 ml. of thiamine, 0.5 of nicotinic acid, 2 grams of activated carbon and 20 grams of sucrose; with 100 ml of coconut water in T2, and 200 ml in T3, obtaining the best result for treatment T1: (M and S Basal, with the addition of 0.4 ml. of thiamine, 0.5 of nicotinic acid, 2 grams of activated carbon and 20 grams of sucrose).
Downloads
References
J. Á. Gómez Ll. & J. Páez. (2005). Tesis: Multiplicación del helecho cacho de venado (Platycerium bifurcatum) in vitro mediante homogeneización de prótalos e in vivo a partir de esporas. Universidad Central de Venezuela. Facultad de Agronomía, p. 96.
H. Hartman, & D. Kester. (1994). Propagación de plantas, principios y prácticas. Tercera reimpresión de la segunda edición. Editorial Continental. México, p. 759.
B. Pérez., A. Mendoza., S. Espinosa & L. D. Gómez. (2010). Gametophyte morphology of Platycerium andinum Baker and Platycerium wandae Racif, Micron 41, pp 806-813. https://doi.org/10.1016/j.micron.2010.05.005
B. León., E. Huamán., J. Roque., M. La Torre., & A. Cano. (2012). Los Helechos Ornamentales en el Perú. Museo de Historia Natural. Primera Edición. Perú, p 50.
R. Vail. Platycerium. Hobbyist's handbook. Library of Congress Cataloging in Publication Data.1ra. Edición, pp. 171.
N. Vázquez., A. Mendoza & B. Pérez. (2012). Morfogénesis de la fase sexual de siete taxa epífitos de Polypodium (Polypodiaceae) de México, Acta Botánica Mexicana, 98, pp 5-21.
M. R. Ramírez & B. Pérez. (1998). Fase gametofítica del helecho Microgramma nítida (Polypodiaceae). Rev. Biol. Trop. 46(3): pp 587-593. https://doi.org/10.15517/rbt.v46i3.20120
B. Pérez-García., A. Mendoza., R. Riba & L. D. Gómez. (2001). Development of the sexual phase of Pseudocolysis bradeorum (Polypodiaceae). Amer. Fern J. 91 pp 214 -226. https://doi.org/10.1640/0002-8444(2001)091[0214:DOTSPO]2.0.CO;2
I. Reyes., B. Pérez & A. Mendoza. (2003). Morfogénesis de los gametofitos de especies mexicanas de Pleopeltis (Polypodiaceae, subfamilia Pleopeltoideae). Rev. Biol. Trop. 51(2): pp 321-332.
E. X. Narváez-Parra., J. H. Jerez & J. E. Mantilla. (2013). Etapas de desarrollo in vitro del gametofito del helecho arborescente Cyathea aff. caracasana (KLOTZSCH). 120, pp 74-84.
L. R. García., D. Torres & C. Romero. (2013). Regeneración de esporofitos de Platycerium bifurcatum (Cav.) C.Chr. a partir de esporas germinadas in vitro, Biotecnología Vegetal 13, pp 99 - 105.
S. Evangelista., S. Escobar., G. Trejo & A. Jiménez. (2001). Propagación in vitro de Platycerium bifurcatum. Memorias del IX Congreso Nacional de Biotecnología y Bioingeniería, XIII Congreso Nacional de Ingeniería Bioquímica y II Congreso Internacional de Ingeniería Bioquímica. Veracruz. México.
C. Morales., S. Montes., V. Paneque & Corbera J. (2003). Aclimatación de vitroplantas de helechos a través del cultivo in vitro de esporas utilizando diferentes sustratos formados a partir de distintos abonos orgánicos. Cultivos Tropicales 24, pp 29-31.
M. E. Tanco., O. G. Martínez & M. L. Bonomo. (2009), Germinación y morfogénesis gametofitica de Pterisinermis (rosenst.) de la Sota. Gayana Bot. 66(1): pp 10-17. https://doi.org/10.4067/S0717-66432009000100002
S. Juntadach. (1996). Studies on spore culture of fern (Platycerium coronarium and Asplenium nidus) in agar medium. Sonklana Karin Journal of Science and Technology 18(3), pp 275-286.
J. Cox., P. Bhatia & N. Ashwath. (2003). In vitro spore germination of the fern Schizaea dichotoma. Scientia Horticulturae 97, pp 369-378. https://doi.org/10.1016/S0304-4238(02)00152-8
H. Fernández & M. A. Revilla. (2003). In vitro culture of ornamental ferns. Plant Cell Tissue and Organ Culture 73, pp 1-13. https://doi.org/10.1023/A:1022650701341
M. R. Ramírez., G. B. Pérez & R. Riba. (2000). El suelo, un banco natural de esporas y helechos. Contacto S 36, pp 15-18.
Schneider HP. Phylogeny and biogeography of the staghorn fern genus Platycerium (Polypodiaceae, Polypodiidae). Am J Bot. 2006; 93(2): 217-225. https://doi.org/10.3732/ajb.93.2.217
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Astriht Ruíz Ríos, Geyden Díaz Montes y Astrid Domy Gutiérrez Ruíz
This work is licensed under a Creative Commons Attribution 4.0 International License.
The works are under a Creative Commons Atribution 4.0 Internacional License
You are free to Share (copy and and redistribute the material in any medium or format) and Adapt the work (remix, transform, and build upon the material) for any purpose, even commercially under the following terms:
Attribution: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
