Evaluación de la susceptibilidad de nematodos gastrointestinales a la ivermectina en equinos del Norte de Veracruz

Maricarmen Gómez-Lorenzo; Melina Maribel Ojeda Chi; Marco Antonio Alarcón Zapata; Dora Romero Salas; Abigail Tabarez Rojas

doi:10.47808/revistabioagro.v12i1.541

Authors

Maricarmen Gómez-Lorenzo Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan.
Melina Maribel Ojeda Chi Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan.
Marco Antonio Alarcón Zapata Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan
Dora Romero Salas Facultad de Medicina Veterinaria y Zootecnia. Universidad Veracruzana.
Abigail Tabarez Rojas Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan.

DOI:

https://doi.org/10.47808/revistabioagro.v12i1.541

Keywords:

helminths, susceptibility, anthelmintic resistance, macrocyclic lactones

Abstract

Cyathostomines are the main gastrointestinal nematodes (GIN) that affect horses; their control is based on the use of anthelmintics (AH); However, resistance to HA has been reported worldwide. Therefore, it is necessary to know the susceptibility of GIN to HA to make efficient treatment decisions. For this reason, the objective of this study was to evaluate the effectiveness of macrocyclic lactones in GIN populations using the fecal egg reduction test and determine the egg reappearance period (ERP). Fifty-eight horses were used in 7 equine units in Tuxpan, Veracruz, a questionnaire was applied to know the general situation of the stables and treatment of each unit. On day zero, feces were collected individually to determine the number of eggs in the feces using the McMaster technique. Animals that tested positive for ≥100 eggs per gram of feces (EPG) were formed into two groups. The treated group received ivermectin (IVM) at a rate of 0.2 mg/kg body weight and the control group did not receive treatment. Samples were taken on days 14-, 42-, and 56-days post-treatment (PT) and EPG was determined. It was observed that 83.3% of the horses were positive for NGI and 1.7% for other nematodes. In six of the units, a 100% reduction in egg count was found and only one unit was suspected of resistance to IVM. Likewise, the ERP was reduced to 42 days. It is concluded that although IVM continues to be effective for the control of NGI in the majority of the units evaluated, however, the ERP has been reduced.

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References

Becher, A.M., van Doorn, D.C., Pfister, K., Kaplan, R.M., Reist, M., Nielsen, M.K. 2018. Equine parasite control and the role of national legislation – A multinational questionnaire survey. Veterinary Parasitology 259:6–12. https://doi.org/10.1016/j.vetpar.2018.07.001 DOI: https://doi.org/10.1016/j.vetpar.2018.07.001

Bucknell D, Hoste H, Gasser RB, Beveridge I. 1996. The structure of the community of strongyloid nematodes of domestic equids. Journal Helminthology. 70(3):185-92. doi: 10.1017/s0022149x0001539x. DOI: https://doi.org/10.1017/S0022149X0001539X

Cain, J.L., Foulk, D., Jedrzejewski, E., Stofanak, H., Nielsen, M.K. 2019. The importance of anthelmintic efficacy monitoring: results of an outreach effort. Parasitology Research. 118:2877–83. DOI: https://doi.org/10.1007/s00436-019-06423-6

Canever, R.J., Braga, P.R.C., Boeckh, A., Grycajuck, M., Bier, D., Molento, M,B. 2013. Lack of Cyathostomin sp. reduction after anthelmintic treatment in horses in Brazil. Veterinary Parasitology 94:35–9. https://doi.org/10.1016/j.vetpar.2012.12.020 DOI: https://doi.org/10.1016/j.vetpar.2012.12.020

Comité Estatal de Información Estadística y Geografía de Veracruz. (2021) Centro de Información Estadística y Geográfica del Estado de Veracruz, Noticias, Cuadernillos Municipales, Álamo Temapache.

Dauparaitė, E., Kupčinskas, T., von Samson-Himmelstjerna, G. et al. 2021. Anthelmintic resistance of horse strongyle nematodes to ivermectin and pyrantel in Lithuania. Acta Veterinaria Scandinavica 63, 5. https://doi.org/10.1186/s13028-021-00569-z DOI: https://doi.org/10.1186/s13028-021-00569-z

Egerton, J.R., Brokken, E.S., Suhayda, D., Eary, C.H., Wooden, J.W., Kilgore, R.L., 1981. The antiparasitic activity of ivermectin in horses. Veterinary Parasitology. 8, 83–88. DOI: https://doi.org/10.1016/0304-4017(81)90020-0

Kaplan, R.M., Nielsen, M.K. 2010. An evidence-based approach to equine parasite control: It ain’t the 60s anymore. Equine Veterinary Education. 22:306–16. DOI: https://doi.org/10.1111/j.2042-3292.2010.00084.x

Kuzmina, T.A., Tolliver, S.C., Lyons. E.T. 2011. Three recently recognized species of cyathostomes (Nematoda: Strongylidae) in equids in Kentucky. Parasitology Research. 108:1179–84. https://doi.org/10.1007/s00436-010-2160-z. DOI: https://doi.org/10.1007/s00436-010-2160-z

Lichtenfels, J.R., Kharchenko, V.A., Dvojnos, G.M. 2008. Illustrated identification keys to strongylid parasites (Strongylidae: Nematoda) of horses, zebras and asses (Equidae). Veterinary Parasitology. 156:4–161. DOI: https://doi.org/10.1016/j.vetpar.2008.04.026

Lyons, E.T., Tolliver, S.C. 2013. Further indication of lowered activity of ivermectin on immature small strongyles in the intestinal lumen of horses on a farm in Central Kentucky. Parasitology Research;112:889–91. https://doi.org/10.1007/s00436-012-3098-0. DOI: https://doi.org/10.1007/s00436-012-3098-0

Matthews, J.B. 2008. An update on cyathostomins: antihelmintic resistance and worm control. Equine Veterinary Education. 20:552–60. DOI: https://doi.org/10.2746/095777308X363912

Mittel, L., Grice, A., Erskine, M., Graves, E., Vaala, W., Tully, R.C., 2012. AAEP Parasite Control Guidelines. AAEP Proc 1–24.

Nielsen, M.K. 2022. Anthelmintic resistance in equine nematodes: Current status and emerging trends, International Journal for Parasitology: Drugs and Drug Resistance. 20, 76-88, https://doi.org/10.1016/j.ijpddr.2022.10.005. DOI: https://doi.org/10.1016/j.ijpddr.2022.10.005

Nielsen, M.K., Branan, M.A., Wiedenheft, A.M., Digianantonio, R., Garber, L.P., Kopral, C.A., Phillippi-Taylor, A.M., Traub-Dargatz, J.L., 2018. Parasite control strategies used by equine owners in the United States: a national survey. Veterinary Parasitology. 250, 45–51 DOI: https://doi.org/10.1016/j.vetpar.2017.12.012

Nielsen, M.K., Mittel, L., Grice, A., Erskine, M., Graves, E., Vaala, W., Tully, R., French, D.D., Bowman, R., Kaplan, R. 2019. AAEP Parasite Control Guidelines. https://aaep.org/wp-content/uploads/2024/02/Internal_Parasite_Guidelines.pdf

Nielsen, M.K., Sauermann, C.W., Leathwick, D.M. 2019. The effect of climate, season, and treatment intensity on anthelmintic resistance in cyathostomins: A modelling exercise. Veterinary Parasitology 269:7–12. https://doi.org/10.1016/j.vetpar.2019.04.003. DOI: https://doi.org/10.1016/j.vetpar.2019.04.003

Nielsen, M.K., von Samson-Himmelstjerna,G., Kuzmina, A.T., van Doorn, D.C.K., Meana, M., Rehbein, S., Elliott, T., Reinemeyer, C.R.. 2022. World Association for the Advancement of Veterinary Parasitology (WAAVP): third edition of guideline for evaluating the efficacy of equine anthelmintics. Veterinary Parasitology. 303, Article 109676 DOI: https://doi.org/10.1016/j.vetpar.2022.109676

Reinemeyer, C.R., Nielsen, M.K. 2009. Parasitism and Colic. Veterinary Clinics of North America: Equine Practice;25:233–45. https://doi.org/10.1016/j.cveq.2009.04.003. DOI: https://doi.org/10.1016/j.cveq.2009.04.003

Sallé, G., Cortet, J., Koch, C., Reigner, F., Cabaret, J.2015. Economic assessment of FEC-based targeted selective drenching in horses. Veterinary Parasitology. 214:159–66. https://doi.org/10.1016/j.vetpar.2015.09.006. DOI: https://doi.org/10.1016/j.vetpar.2015.09.006

Sauermann, C.W., Nielsen, M.K., Luo, D., Leathwick, D.M. 2019. Modelling the development of anthelmintic resistance in cyathostomin parasites: The importance of genetic and fitness parameters. Veterinary Parasitology, 269:28–33. https://doi.org/10.1016/j.vetpar.2019.04.007. DOI: https://doi.org/10.1016/j.vetpar.2019.04.007

Stratford, C.H., Lester, H.E., Pickles, K.J., McGorum, B.C., Matthews, J.B., 2014. An investigation of anthelmintic efficacy against strongyles on equine yards in Scotland. Equine Veterinary Journal. 46, 17–24. DOI: https://doi.org/10.1111/evj.12079

Sutherland, I.A., Leathwick, D.M., 2011. Anthelmintic resistance in nematode parasites of cattle: a global issue? Trends Parasitology. 27, 176–181. DOI: https://doi.org/10.1016/j.pt.2010.11.008

Traversa, D., von Samson-Himmelstjerna, G., Demeler, J., Milillo, P., Schürmann, S., Barnes, H., Otranto, D., Perrucci, S., di Regalbono, A.F., Beraldo, P., Boeckh, A., Cobb, R. 2009. Anthelmintic resistance in cyathostomin populations from horse yards in Italy, United Kingdom and Germany. Parasites and Vectors 2009;2:1–7. https://doi.org/10.1186/1756-3305-2-S2-S2. DOI: https://doi.org/10.1186/1756-3305-2-S2-S2

Wagner, E.L., Tyler, P.J. 2011. A Comparison of weight estimation methods in adult horses. Journal of Equine Veterinary Science. 31:706–10. https://doi.org/10.1016/j.jevs.2011.05.002. DOI: https://doi.org/10.1016/j.jevs.2011.05.002

Wyk, J.A. 2001. Refugia-overlooked as perhaps the most potent factor concerning the development of anthelmintic resistance. Onderstepoort Journal of Veterinary Research. 68:55–67.