Disinfection of nodal segments in the in vital establishment of Erythrina americana Miller.
DOI:
https://doi.org/10.47808/revistabioagro.v5i1.87Keywords:
in vitro, nodal segments, micropropagationAbstract
In order to establish an in vitro multiplication and disinfection protocol for the production of American Erythrina Miller seedlings, two different disinfectants ethanol and sodium hypochlorite were evaluated in percentage concentrations of 35, 45, 55, 65 and 70 (v / v) and 20, 25, 30, 35 and 40 respectively, with an immersion time of 5, 10, 15, 20 and 25 minutes for both; 2.0 cm long nodal segments were used. The culture medium used was Murashige and Skoog (MS) added with 3% sucrose, vitamins and BAP 0.5 mgL-1 and AIB 0.1 mgL-1, a yolk was stimulated during the multiplication stage, which indicates that it is necessary to make more combinations of growth regulators in order to obtain the optimum concentration that stimulate the greatest number of possible buds. The results obtained showed that micropropagation of this species is a viable alternative for the commercialization, conservation and reforestation of degraded areas.
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Copyright (c) 2017 María de Jesús Martínez Hernández , Sandra Ivonne Sánchez Hernández , Doris Castillo Rocha , Mauricio Luna Rodríguez , Mauricio Luna Rodríguez
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