Effect of the addition of an antioxidant on the mitochondrial activity and motility of cryopreserved bovine spermatozoa
DOI:
https://doi.org/10.47808/revistabioagro.v10i2.429Keywords:
Antioxidant, spermatozoa, cryopresevationAbstract
The freeze-thaw process causes oxidative stress, osmotic stress, heat shock, intracellular ice formation, alterations in the composition of plasma membrane lipids and proteins, decreased sperm viability and motility, damage to the mitochondria, acrosome and tail, and promotes DNA fragmentation. Mitochondria are a source of energy for respiration and the main site of reactive oxygen species (ROS) generation. Antioxidants must be present within the mitochondrion in large quantities to be effective in reducing ROS. However, the mitochondrial inner membrane is not permeable to most molecules. Several studies indicate that antioxidant supplementation during the
semen freezing process improves frozen-thawed sperm quality to several degrees, but there is still a lack of a recognized effective antioxidant. There are few studies on the effects of supplementation of a mitochondria-targeted antioxidant in semen cryopreservation, so the aim of the present work is to evaluate the effect of the addition of MitoTEMPO on the mitochondrial activity and motility of bovine sperm cryopreserved in a commercial extender. The supplementation of the antioxidant in the commercial extender at different concentrations improved sperm motility by 13% and 17% with respect to the control group; decreased ROS by 29% and 18% and increased the percentage of ATP production by 257% and 161% in the frozen-thawed bovine spermatozoa. These results support the importance of the use of antioxidants during sperm cryopreservation, concluding that the use of MitoTEMPO in relatively low concentrations in the extender improves semen quality after sperm thawing.
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