Nematicidal and fungicidal effect of hydroalcoholic extracts of Piper lapathifolium and P. melastomoides
DOI:
https://doi.org/10.47808/revistabioagro.v6i2.Especial.252Keywords:
fusarium, panagrellus redivivus, biocidalAbstract
The Piper has been shown to have biocidal effects on different organisms harmful to agriculture such as phytopathogenic nematodes and fungi. Because most of these organisms are controlled or erradicated with toxic chemical compounds, less toxic alternative have been sought to replace them, included natural extracts, such as those of Piper. In this sense, the present work evaluated the namaticidal and fungicidal effect of the hydroalcoholic extracts (ETOH: H2O 7: 3) of Piper lapathifolium and P. melastomoides. The nematicidal effect was determined in Panagrellus ridivivus in a 24-hour trial and the Mean Lethal Concentration (LC50) was estimated. The fungicidal effect was evaluated in Fusarium oxysporum and F. solani, by the plate dilution test. The Average Effective Concentration (EC50) was shown by the Probit model. The results showed that P. melastomoides presented a nematicidal effect at levels lower than 5 mg / ml with an LC50 of 2.32 mg / ml, in the case of P. lapathifolium it did not present activity in the nematodes, in the case of the fungicidal activity this extract (EC50: 26.32 and 5.83 mg / mL), whereas P. melastomoides exhibited lower activity. It is concluded that P. melastomoides has a nematicidal effect and P. lapathifolium fungicide.
Downloads
References
Agyare, C., Spiegler, V., Sarkodie, H., Asase, A., Liebau, E. y Hensel, A. 2014. An ethnopharmacological survey and in vitro confirmation of the ethnopharmacological use of medicinal plants as anthelmintic remedies in the Ashanti region, in the central part of Ghana. Journal of Ethnopharmacology 158 PART A: 255-263. https://doi.org/10.1016/j.jep.2014.10.029
Atjanasuppat, K., Wongkham, W., Meepowpan, P., Kittakoop, P., Sobhon, P., Bartlett, A. y Whitfield, P. J. 2009. In vitro screening for anthelmintic and antitumour activity of ethnomedicinal plants from Thailand. Journal of Ethnopharmacology 123(3): 475-482. https://doi.org/10.1016/j.jep.2009.03.010
Brenner, S. 1974. The genetics of Caenorhabditis elegans. Genetics 77(1):71-94. https://doi.org/10.1093/genetics/77.1.71
Carmona-Hernández, Ó., del Socorro Fernández, M., Palmeros-Sánchez, B. y Lozada-García, J. A. 2014. Actividad insecticida de extractos etanólicos foliares de nueve piperáceas (Piper spp.) en Drosophila melanogaster. Revista Internacional de Contaminación Ambiental 30: 67-73.
Carmona-Hernández, O., Lozada-García, J. A., Martínez-Hernández, M. J., Fernández, M. S. y Torres-Pelayo, V. R. 2016. Piper L. genus potential as natural biocide. Wulfenia Journal 23(6): 65-95.
Domínguez, A. X. 1979. Métodos de Investigación Fitoquímica. Editorial Limusa, México, D.F.
Finney, D. L. 1987. Chapter 18: Assay base don quantal responses. Statistical Method in Biological Assay 469-490.
Juárez-Becerra, G. P., Sosa-Morales, M. E. y López-Malo, A. 2010. Hongos fitoptógenos de alta importancia económica: descripción y métodos de control. Temas Selectos de Ingeniería de Alimentos 4(2): 14-23.
Jensen, H. R., Scott, I. M., Sims, S., Trudeau, V. L. y Arnason, J. T. 2006. Gene expression profiles of Drosophila melanogaster exposed to an insecticidal extract of Piper nigrum. Journal of Agricultural and Food Chemistry 54(4):1289-1295. https://doi.org/10.1021/jf052046n
Johnson, S. B., Olsen, N., Rosen, C. y Spooner, D. M. 2010. Commercial potato production in North America. American Journal of Potato Research 87 suppl: 1-90.
Katiki, L. M., Ferreira, J. F. S., Zajac, A. M., Masler, C., Lindsay, D. S., Chagas, A. C. S. y Amarante, A. F. T. 2011. Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. Veterinary Parasitology 182(2-4): 264-268. https://doi.org/10.1016/j.vetpar.2011.05.020
Kusuma, S. A. F., Tjitraresmi, A. y Susanti, G.2017. Antibacterial effect of red Piper betel leaf (Piper crocatum ruiz & amp; pav.) ethanol extracts to Lactobacillus acidophilus and L. bifidus growth inhibition. Asian Journal of Pharmaceutical and Clinical Research 10(14): 65. https://doi.org/10.22159/ajpcr.2017.v10s2.19490
Lee, S. E., Park, B. S., Kim, M. K., Choi, W. S., Kim, H. T., Cho, K. Y., Lee. S. G. y Lee, H. S. 2001. Fungicidal activity of pipernonaline, a piperidine alkaloid derived from long pepper, Piper longum L., against phytopathogenic fungi. Crop Protection 20(6): 523-528. https://doi.org/10.1016/S0261-2194(00)00172-1
Moreno, M. E., González, S., Acevedo, L., Morales, G., Betancur, M, López, J. J. y Peláez, C. A. 2000. Drosophila melanogaster (Diptera: Drosophilidae): modelo biológico para la estandarización de extractos naturales con actividad insecticida (El Neem- Azadirachta indica- un caso particular). Revista Entomológica de Colombia 26 (1-2): 21-55.4
Neela, F. A., Sonia, I. A. y Shamsi, S. 2014.Antifungal activity of selected medicinal plant extract on Fusarium oxysporum Schlecht the causal agent of fusarium wilt disease in tomato. American Journal of Plant Sciences 05(18): 2665-2671. https://doi.org/10.4236/ajps.2014.518281
Ngurah, D. y Oshawa, K. 2007. Fungicidal activity of Piper betle extract against Fusarium oxysporum f. sp. Vanilla. JISSAAS 13(2): 40-46.
Parmar, V. S., Jain, S. C., Bisht, K. S., Jain, R., Taneja, P., Jha, A., Om, D., Tyagi, A. K., Prasad, J., Wengel, T C., Olsen, E. y Per, M. B. Phytochemistry of genus Piper. Phytochemistry 46(4): 597-673. https://doi.org/10.1016/S0031-9422(97)00328-2
Pica, G. Y. 2008. Ensayo de toxicidad con el nemátodo Panagrellus redivivus. Ensayos Toxicológicos para la Evaluación de Sustancias Químicas en Agua y Suelo 139-154
Roh, J. Y. y Choi, J. 2008. Ecotoxicological evaluation of chlorpyrifos exposure on the nematode Caenorhabditis elegans. Ecotoxicology and Environmental Safety 71(2): 483-489. https://doi.org/10.1016/j.ecoenv.2007.11.007
Scott, I. M., Jensen, H. R., Philogène, B. J. R. y Arnason, J. T. 2008. A review of Piper spp. (Piperaceae) phytochemistry, insecticidal activity and mode of action. Phytochemistry Reviews 7(1): 65-75. https://doi.org/10.1007/s11101-006-9058-5
Singha, I. M., Kakoty, Y., Unni, B. G., Kalita, M. C., Das, J., Naglot, A., Wann S. B. y Singh, L. 2011. Control of Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici using leaf extract of Piper betle L.: A preliminary study. World Journal of Microbiology and Biotechnology 27(11): 2583-2589. https://doi.org/10.1007/s11274-011-0730-6
Soberón, G. V., Rojas, C., Saavedra, J., Kato, M. J. y Delgado, G. E. 2006. Acción biocida de plantas de Piper tuberculatum Jacq. sobre Diatrea saccharalis (Lepidóptera, Pyralidae). Revista Peruana de Biología 13(1): 107-112. https://doi.org/10.15381/rpb.v13i1.1770
Sosa, V. y Gómez-Pompa, A. 1994. Lista florística del estado de Veracruz. Flora de Veracruz 82: 1-245.
Tangarife-Castaño, V., Correa-Royero, J. B., Roa-Linares, V. C., Pino-Benitez, N., Betancur-Galvis, L. A., Durán, D. C., Staschenko, E. E. y Mesa-Arango, A. C. 2014. Anti-dermatophyte, anti-Fusarium and cytotoxic activity of essential oils and plant extracts of Piper genus. Journal of Essential Oil Research 26(3):221-227. https://doi.org/10.1080/10412905.2014.882279
Varsha, H. y Sonali, S. 2014. Studies on qualitative phytochemical analysis of selected species of Piper. International Journal of Life Sciences Special Issue A2: 156-158
Villaseñor, J. L. 2016. Checklist of the native vascular plants of Mexico. Revista Mexicana de Biodiversidad 87(3): 559-902. https://doi.org/10.1016/j.rmb.2016.06.017
Wiratno, T. D., Van den Berg, H., Riksen, J. A. G., Rietjens, I. M. C. M., Djiwanti, S. R. y Murk, A. J. 2009. Nematicidal activity of plant extracts against the root-knot nematode, Meloidogyne incognita. The Open Natural Products Journal 2:77-85. https://doi.org/10.2174/1874848100902010077
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Oscar Carmona Hernández, José Armando Lozada García, María del Socorro Fernández, Mauricio Luna Rodríguez, María de Jesús Martínez Hernández y José Antonio Guerrero Analco
This work is licensed under a Creative Commons Attribution 4.0 International License.
The works are under a Creative Commons Atribution 4.0 Internacional License
You are free to Share (copy and and redistribute the material in any medium or format) and Adapt the work (remix, transform, and build upon the material) for any purpose, even commercially under the following terms:
Attribution: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
