In vitro management alternatives of Erwinia spp. pathogenic in Saintpaulia ionantha
DOI:
https://doi.org/10.47808/revistabioagro.v5i2.103Keywords:
african violet, bactericides, tuxpanAbstract
The objective of the work was to evaluate the in vitro efficacy of different bactericides for the control of Erwinia sp. isolated on African violet plants (Saintpaulia ionantha) from a greenhouse in the town of Tenango de las Flores, Huachinango, Puebla. After realizing the postulates of Koch, the causal agent of the bacterial blight was identified as Erwinia sp. This pathogen mainly damages the leaves and can destroy the whole plant since it produces large amounts of pectolytic enzymes. In the laboratory bioassay, a completely randomized design was used with five repetitions and seven treatments, Oxicrop, Agri-Mycin® 100, Bactrol® 2x, Hidrobacter®, Terramycin® Agricola 5%, Final Bacter and Agrobacter®, adding a control without bactericide and evaluating the effect of halos of inhibition produced in a trypticasein soy agar medium. The statistical analysis indicated statistically significant differences between treatments, 48 hours after having formed inhibition halos for each treatment (ANOVA p <=0.05). The comparison of means test showed the existence of significant differences regarding the halos of inhibition caused in each of the treatments under study, being the treatment 4 (Hidrobacter®), the one that presented the highest halo of inhibition being statistically similar to treatments 1 (Oxicrop) and 6 (Final Bacter). Treatment 2 (Agri-Mycin® 100) showed the lowest halo of inhibition which means that the bacterial isolate showed tolerance to the effect of the active ingredients (streptomycin and oxytetracycline) contained in the commercial product and its use in the field would not be recommended. the recommended doses.
Downloads
References
Chinedum E. 2005. Microbial resistance to antibiotics.Afr. J. Biotechnol. 4 (13), 1606-1611.
https://doi.org/10.4314/ajfand.v4i13.71776
Cunha, J.F., E. A. Toledo-Picoli., A. Couto- Alfenas and R. Coelho-Gonçalves. 2006. "In vitro" effect of antibiotics and rhizobacteria on the control of phytopathogenic bacteria in Eucalyptus spp. Rev. Ávore. 30:1-9.
https://doi.org/10.1590/S0100-67622006000600001
Espinoza, R. A., Silva, P. J., González, P. O., Fajardo, R. L. y Pérez, P. J. (2007). Multiplicación in vitro de Violeta Africana (Saintpaulia ionantha). Revista Electrónica Gramma Ciencia.
Farfán, L. M., Benítez S. V., y Hoyos-Carvajal, L. M. 2014. Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos. Revista Colombiana de Ciencias Hortícolas 8:20-33.
https://doi.org/10.17584/rcch.2014v8i1.2797
Faria G. T y Maringoni A. C. 2000. Ação de produtos químicos in vitro e in vivo sobre Clavibacter michiganensis subsp. michiganensis, agente causal do cancro bacteriano do tomateiro. Scientia Agricola. 57(3): 439-443.
https://doi.org/10.1590/S0103-90162000000300011
García G., Hernández C. y Martínez L. 1999. Floricultura en México y entorno mundial. Proyecciones, Año 1 Número 1 junio-agosto de 1999. http://www.cem.itesm.mx/dacs/publicac iones/proy/n1/inveco1.html. Consultado 23 de febrero del 2017.
Gilchrist-Saavedra, L., Fuentes-Dávila, G., Martínez-Cano, C, M LópezAtilano, R.M., Duveiller, E., Singh, R. P., Henry, M., and García, A. I. 2005. Guía práctica para la identificación de algunas enfermedades de trigo y cebada. 2ª edición. CYMMYT. México. 68 págs.
González, C. J. C., Elorza, M. P., López, J. A., Mateos R. R. A., y González, A. A. 2013. Etiología de la pudrición foliar blanda de la Violeta Africana (Saintpaulia ionantha Wendlan) en Tenango de las flores, Huachinango, Puebla. Revista Científica Biológico Agropecuaria Tuxpan 1:22-26.
Jorgensen J. H. and Ferraro M. J. 1998. Antimicrobial susceptibility testing: general principles and contemporary practices. Clinical Infectious Diseases.26:973-980.
https://doi.org/10.1086/513938
Kunz L. J. y Moellering R. C. 1971. Mechanical method of inoculating plates for antibiotic sensitivity testing. Applied microbiology. 22(3): 476-477.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Marisol Félix Reyes , Pablo Elorza Martínez , Arianna Rubí González Sánchez
This work is licensed under a Creative Commons Attribution 4.0 International License.
The works are under a Creative Commons Atribution 4.0 Internacional License
You are free to Share (copy and and redistribute the material in any medium or format) and Adapt the work (remix, transform, and build upon the material) for any purpose, even commercially under the following terms:
Attribution: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.